Keck Microscopy Center
Purpose
The W. M. Keck Microscopy Center provides light microscopy and image analysis services to the University of Washington (UW) research community as a recharge center. External, non-UW users may also use the Keck Center.
The Keck Center manager, Dr. Nathaniel Peters, provides equipment training, experimental consultation and support, equipment demos, and assisted imaging for one-time or limited basis needs.
Description
Keck Center instrumentation includes laser-scanning confocal microscopes, widefield microscopes, and an STF-funded advanced IMARIS image analysis workstation that is FREE for all UW students.
The Keck Center operates as a UW recharge center, and is supported by the departments of Physiology and Biophysics (PBio), Pharmacology, Genome Sciences, and Laboratory Medicine & Pathology (DLMP). It was established in 1990 as the W. M. Keck Center for Advanced Studies in Neural Signaling through a grant from the Keck Foundation so that researchers in the Department of Physiology and Biophysics (PBio) and the Department of Pharmacology could explore how nerve cells acquire, store and transmit information.
Type
Equipment
- 2 Laser-scanning Confocal Microscopes: Leica SP8X and Zeiss 710
- 2 Widefield Microscopes: Leica DMI6000 and Nikon E600
- IMARIS Image Analysis Software (Full license with tech support)
- Environmental Control Equipment (For live-imaging)
Capabilities
- Confocal: 3D & 4D (Timelapse) scanning, Mosaic tile-scanning, Multi-point live imaging, FRAP and FRET imaging, Resonant scanning, Sequential scanning of up to 5 colors, Spectral scanning, Reflection scanning, LIGHTNING deconvolution for Super-Resolution confocal imaging
- Widefield: Multi-color fluorescence imaging, Brightfield, DIC, Phase Contrast, and RGB-color imaging, Z-series, Timelapse imaging, Mosaic tiling, Multi-point live imaging, Extended depth-of-field image processing
- Image Analysis: Surface and Spot Analysis, Particle Tracking and Lineage, Co-localization, Vantage Plotting, XT capability, Cell Analysis, Filament (neuron & vessel) Tracer, Deconvolution Processing, Batch Processing, Animation
Keywords: General
Microscope, Optical Microscopy, Confocal Microscopy, Widefield Microscopy, Image Analysis
Keywords: Specific
Laser-Scanning Confocal Microscope, Widefield Microscope, Fluorescence, Epifluorescence, Brightfield, Darkfield, Phase Contrast, Differential Interference Contrast, DIC, Focus Stacking, Mosaic Tiling, Z-series, Color Camera, Histology, Fluorescence Camera, Immunofluorescence, Immunohistochemistry, in situ Hybridization, Fluorescence in situ Hybridization, FISH, FRAP, FRET, Resonant Scanner, Spectral Scanner, Reflection Scanner, Live Imaging, IMARIS, Deconvolution, Co-localization, Animation
Type of Resource
Light Microscopy and Image Analysis
